%0 Journal Article
%T Studies on expression and biochemical characteristics of single chain fragment variable A1 against White Spot Syndrome Virus of shrimp<br>抗对虾白斑综合症病毒的单链抗体A1的表达和生物化学特性
%A ZHANG Xiao-Hua
%A DAI Ling-Fen
%A DAI He-Ping
%A <br>张晓华
%A 戴玲芬
%A 戴和平
%J 水生生物学报
%D 2006
%I 
%X 用噬菌体展示技术制备了抗对虾白斑综合症病毒(WSSV)的单链抗体A1。该抗体在30℃培养条件下诱导表达20h后,其蛋白表达量可达总菌体蛋白的3.67%。用亲和层析柱和SephadexG-100层析柱可将单链抗体A1纯化为一条单电泳条带,其分子量约为31.5kD。用等电聚焦电泳测定,其等电点为pH5.8。ELISA测定表明冻干的单链抗体A1在室温储藏4年后与WSSV结合仍具有较高的活力。
%K Single chain fragment variable(scFv)
%K White Spot Syndrome Virus(WSSV)
%K Shrimp<br>单链抗体
%K 白斑综合症病毒(WSSV)
%K 对虾
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=256651267D5D2E4520A7707D05E98747&aid=69A3966BED748DE5&yid=37904DC365DD7266&vid=340AC2BF8E7AB4FD&iid=0B39A22176CE99FB&sid=A8DE7703CC9E390F&eid=769BD58726D66E7D&journal_id=1000-3207&journal_name=水生生物学报&referenced_num=4&reference_num=4