%0 Journal Article
%T Simultaneous Determination of Tetrahydropalmatine, Dehydrocorydaline, Berberine and Palmatine in Dog Plasma by LC-MS/MS: Application to Pharmacokinetic Study of Rhizoma Corydalis Extraction<br>LC-MS/MS法同时测定犬血浆中四氢巴马汀、脱氢紫堇碱、小檗碱和巴马汀及其在元胡提取物的药代动力学研究应用
%A Lin Li
%A Liu Jianxun
%A Zhang Ying
%A Li Xinzhi
%A Duan Cangling
%A Lin Chengren
%A Li Lei
%A <br>林力
%A 刘建勋
%A 张颖
%A 李欣志
%A 段昌令
%A 林成仁
%A 李磊
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X A sensitive liquid chromatography-tandem mass spectrometry(LC-MS/MS) was developed and validated for the simultaneous quantification of tetrahydropalmatine(THP),dehydrocorydaline(DHC),berberine(Ber) and palmatine(Pal) in dog plasma.The compounds were pretreated by protein precipitation using organic solvent.Chromatographic separation was achieved on a reversed-phase C18 column with the mobile phase of acetonitrile-water(0.1% acetic acid) and step gradient elution resulted at a flow rate of 0.60 mL·min-1.A tandem mass spectrometer equipped with electrospray ionization source was used as detector and operated in the positive ion mode.Quantification was performed using multiple reaction monitoring(MRM) of the transitions m/z 366.2/350.2(THP),356.2/191.2(DHC),336.2/320.2(Ber) and 352.2/336.2(Pal).The method showed excellent linearity over the concentration range of 0.0488-50 μg·L-1 for DHC,THP,Ber and 0.0488-100 μg·L-1 for Pal.The low limits of quantification were at 0.0488 μg·L-1.The precision,accuracy and stability of the analytes meet the requirements.The method was sensitive,accurate and rapid.It can be applied in the determination of plasma concentration of four alkaloids.
%K Rhizoma Corydalis
%K pharmacokinetics
%K LC-MS/MS<br>元胡
%K 药代动力学
%K 液质联用
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=9B3C227A81E2A28C47495110A2909420&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=6DC9B9BB39B3E540&eid=145178081E5EA3CE&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0