%0 Journal Article %T Study on Protein Detection Method in Qingkailing Injection
清开灵注射液中杂蛋白的检测方法研究 %A Wang Xintong %A Tang Siyuan %A Lu Jianqiu %A Shi Xinyuan %A
王新彤 %A 唐思园 %A 卢建秋 %A 史新元 %J 世界科学技术-中医药现代化 %D 2012 %I %X This study was aimed to establish a method for determining protein content in Qingkailing Injection. Biuret colorimetry was used to determine the protein which had been precipitated by trichloroacetic acid. SDSpolyacrylamide gel electrophoresis (SDS-PAGE) was used to determine the molecular weight of the protein. The results showed that molecular weight of protein in Qingkailing Injection is between 3 KD to 7 KD. It was concluded that SDS-PAGE can be used to determine the protein in Qingkailing Injection. This method is simple and rapid. %K Qingkailing Injection %K protein %K SDS-PAGE
清开灵注射液 %K 杂蛋白 %K SDS-PAGE %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=5DAB06808AFD0BA3D55C61E1EEC02597&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=38B194292C032A66&sid=8D0822DF60ACC08F&eid=9E8E868F1632249A&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0