%0 Journal Article
%T Optimization on Protein-removing Technology of Ginseng Polysaccharides by Orthogonal Test<br>正交试验优选人参多糖除蛋白工艺
%A Song Lihua
%A Xiao Wei
%A <br>宋利华
%A 萧 伟
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X This study was aimed to optimize conditions for protein-removing from ginseng polysaccharides.The protein-removing rate,retaining rate of polysaccharides and uronic acid were used as indicators.Results of two protein-removing methods(i.e.,Sevag method and TCA method) were compared.And the optimized protein-removing conditions were obtained by orthogonal test.The results of Sevag method were better than TCA method.The optimized condition for protein-removing from ginseng polysaccharides was that Sevag reagent volume was equal to 1/3 times of 5% ginseng polysaccharides solution.And the volume ratio of CHCl3 and normal butanol was 4∶1.The protein-removing process was conducted 6 times.It was concluded that the method is efficient and can be used for the separation and refinement of ginseng polysaccharides.
%K Ginseng polysaccharides
%K protein-removing
%K orthogonal test<br>人参多糖
%K 除蛋白
%K 正交试验
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=4561954BE0A0F99D626AA55C43446E0A&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=0B39A22176CE99FB&sid=BD9BFF5428C0CED6&eid=5D9E227628843A85&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0