%0 Journal Article
%T Analysis of rDNA ITS Sequence of Wild Dendrobium Officinale<br>野生铁皮石斛rDNA ITS序列分析
%A Li Haixi
%A Huang Haibo
%A Liu Peng
%A Huang Song
%A Zhang Guifang
%A Zhang Danyan
%A Zhang Jianxi
%A Chen Nian
%A Lai Xiaoping
%A <br>李海霞
%A 黄海波
%A 刘鹏
%A 黄松
%A 张桂芳
%A 张丹雁
%A 张建霞
%A 陈念
%A 赖小平
%J 世界科学技术-中医药现代化
%D 2012
%I 
%X This study was aimed to determine the sequence and analyze rDNA ITS sequences of samples of wild Dendrobium officinale from different origins. The feasibility of using ITS sequence to identify the origin of Dendrobium officinale was discussed. Total DNA was extracted and PCR was applied by using primer ITS5F/ITS4R. Then the PCR products are electrophoresised, purified, sequenced and submitted to GenBank. Clustal W was used to compare the multiple alignment of all 33 ITS sequences, including 11 submitted sequences in this article. Dendrobium nobile and Bulbophyllum odoratissimum were used as outgroup to analyze phylogeny by building neighbor-joining tree. The results showed that 11 sequences were selected to be submitted to the GenBank from all sequences. The achieved accession number was JF803235-JF803245. A total of 645 homologous sites, 9 informative sites (3 sites of ITS1, 4 sites of 5.8S, and 2 sites of ITS2) were obtained. Phylogenetic analysis showed that Dendrobium nobile and Bulbophyllum odoratissimum were in the base of the tree, and the other 32 Dendrobium officinale together were formed on large branches. No correspondence between the origin and the topology of phylogenetic tree were founded. It was concluded that due to the current lack of comprehensive standard of Dendrobium officinale samples and ITS reference sequence, only using ITS sequences cannot get enough phylogenetic information sites. Therefore, it is not feasible to speculate the origin of Dendrobium officinale.
%K Dendrobium officinale
%K ITS
%K phylogenetic analysis
%K species identification<br>铁皮石斛
%K 内转录间隔区
%K 系统发育分析
%K 物种鉴定
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=A9DB1C13C87CE289EA38239A9433C9DC&cid=B5F35A1FC27C1FF7&jid=44257A35316DB0727BA6BC707B9EDAAB&aid=08F5EC0AA8616491C2DE2238C0B65A33&yid=99E9153A83D4CB11&vid=F3583C8E78166B9E&iid=94C357A881DFC066&sid=8C654DF4DA4F6E06&eid=53A9347E622951C1&journal_id=1674-3849&journal_name=世界科学技术-中医药现代化&referenced_num=0&reference_num=0