%0 Journal Article
%T Prokaryotic expression of a glycosyltransferase Med-ORF8 involved in an antitumor antibiotic medermycin biosynthesis aided by the molecular chaperone<br>分子伴侣协助下抗肿瘤抗生素美达霉素生物合成中的糖基转移酶Med-ORF8 的原核表达
%A WAN Juan
%A DENG Hui-Qun
%A ZHANG Bi-Qian
%A WANG Wei
%A CAI Xiao-Feng
%A LI Ai-Ying
%A <br>万娟
%A 邓会群
%A 张碧乾
%A 王蔚
%A 蔡晓凤
%A 李爱英
%J 微生物学通报
%D 2011
%I 
%X Glycosyltransferases play central roles in the glycosylation of biosynthetic pathways of many antibiotics. The prokaryotic expression and enzymatic features of Med-ORF8, a glycosyltransferase involved in the biosynthesis of medermycin with strong antitumor activity, still remains obscure. Here, firstly, we performed the computer modeling of the 3-D structure of Med-ORF8 to prove that the presence of 6*His-tag at the terminals of Med-ORF8 had no effect on its 3-D structure. Subsequently, we established two prokaryotic expression systems with pET vectors to express Med-ORF8, and found that pET-28a (+) could gain a higher yield of the target protein than pET-23a (+), but mostly in an insoluble form. Finally, we introduced a molecular chaperone gene into the system with pET-28a (+) and found that the co-expression of the molecular chaperone with Med-ORF8 could efficiently decrease the formation of the inclusion body and increase the accumulation of soluble Med-ORF8.
%K Glycosylation
%K Medermycin
%K Glycosyltransferase
%K Prokaryotic expression
%K Molecular chaperone<br>糖基化
%K 美达霉素
%K 糖基转移酶
%K 原核表达
%K 分子伴侣
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=A876FC35BCF62E2A4BAB640D9AE22C69&yid=9377ED8094509821&vid=16D8618C6164A3ED&iid=0B39A22176CE99FB&sid=78F0EFE028BD3783&eid=0DEB7A8A66C33AAD&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=20