%0 Journal Article
%T Influence of different induction methods on Agrobacterium tumefaciens-mediated transformation efficiency of Trichoderma<br>不同诱导方法对农杆菌介导的木霉菌遗传转化效率的影响
%A QU Lian-Hai
%A YANG Qian
%A <br>曲连海
%A 杨谦
%J 微生物学通报
%D 2013
%I 
%X Objective] To determine the optimum induction method, different induction methods on Agrobacterium tumefaciens EHA105-mediated transformation system of Trichoderma harzianum T88 were investigated. Methods] By comparing the conversion efficiency of double cultivation, blotting cultivation and liquid co-cultivation, the optimum induction method can be determined. Results] The conversion efficiency of double cultivation is about 10 transformants per 107 conidia, the conversion efficiency of blotting cultivation is about 20 transformants per 107 conidia, while the conversion efficiency of liquid co-cultivation is about 100 transformants per 107 conidia. Conclusion] The conversion efficiency of liquid co-cultivation is the maximum in A.tumefaciens-mediated transformation system of T. harzianum, it is about 5?10 times of double cultivation and blotting cultivation.
%K Agrobacterium Tumefaciens
%K Trichoderma harzianum
%K conversion efficiency<br>根癌农杆菌
%K 哈茨木霉
%K 转化效率
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=78024727B5F4EF6AA9CA8E605B5FC464&aid=9C65539B20E123FC9EC509A8F04942E5&yid=FF7AA908D58E97FA&vid=1371F55DA51B6E64&iid=0B39A22176CE99FB&sid=6826CBE9C80ACB20&eid=B4E8EA49DAAEB84F&journal_id=0253-2654&journal_name=微生物学通报&referenced_num=0&reference_num=0