%0 Journal Article
%T Advances in approaches for the quantitative detection of microRNAs<br>MicroRNA定量检测方法的研究进展
%A JING Hua
%A SONG Qin-Xin
%A ZHOU Guo-Hua
%A <br>景花
%A 宋沁馨
%A 周国华
%J 遗传
%D 2010
%I 
%X MicroRNAs (miRNAs) are a class of endogenous non-coding RNAs that play an important role in the control of developmental process of different cells by negative regulation of protein-coding gene expression. Analyzing miRNA expression in tissues or cells can supply valuable information for investigating the biological function of these molecules. Recently, researchers had proposed a number of approaches for analyzing the differences of miRNA expression among dif-ferent physiological or pathological conditions, and found that aberrant expression of miRNA was related to cancers, neu-rological disorders and heart diseases, etc. This review focuses on newly developed strategies for miRNA quantification, and elucidates in detail the probe-hybridization based methods including Northern blotting, microarray, gold nanoparticle labelling, and splinted ligation with radioactive labels. The amplification-based methods including quantitative PCR, rolling cycle amplification, invader assay, and the next generation sequencing methods were also discussed. The advantages and disadvantages of these methods were compared.
%K microRNA
%K microRNA
%K quantitative detection
%K research progress<br>microRNA
%K 定量检测
%K 研究进展
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=3E23F50E071DF18E21B2F5AEE4F1FA5E&aid=A2D75BF2DC133D0B9DE391612E1D8FB5&yid=140ECF96957D60B2&vid=9971A5E270697F23&iid=CA4FD0336C81A37A&sid=4AD960B5AD2D111A&eid=1371F55DA51B6E64&journal_id=0253-9772&journal_name=遗传&referenced_num=1&reference_num=60