%0 Journal Article
%T Application of Cryo-sectioning Technique in Weed Fruits<br>杂草果实的冰冻切片技术
%A Ruhai Li
%A Sheng Qiang
%A <br>李儒海
%A 强胜
%J 植物学报
%D 2010
%I 
%X A fast, simple and effective technique for weed fruit cryo-sectioning is introduced. Highly lignified fruits of 4 weed species that can float on water were selected to be dissected by a cryo-sectioning technique so that the internal structure adaptive to floating could be clearly observed. The method includes six steps: material fixation, pre-treatment, fixing to cold stage, cryo-sectioning, section flattening, stain and observation and photography. The successful application of cryo-sectioning technique to weed fruit anatomy demonstrated its advantage without a long process of softening, dehydration and embedding. The crucial steps of the technique are to select a proper concentration of glycerin, a suitable temperature of freezing and an appropriate way of section flattening. In this study, the better micrographs could be obtained when the weed fruits were pretreated with 15% glycerin.
%K cryo-sectioning
%K microstructure
%K weed fruits<br>冰冻切片
%K 显微结构
%K 杂草果实
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=35F0005BC26AD3DCE134CF5D5E34B066&yid=140ECF96957D60B2&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=547991B346A6585E&eid=6EDA906E07280FB0&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0