%0 Journal Article
%T Construction of a Suppression Subtractive Hybridization Library for Stylosanthes guianensis Under Aluminum Stress and Expressed Sequence Tag Analysis<br>铝胁迫下柱花草SSH文库构建及表达序列标签分析
%A Qifeng Wang
%A Qiong Yi
%A Kunzhi Li
%A Limei Chen
%A Yongxiong Yu
%A <br>王奇峰
%A 易琼
%A 李昆志
%A 陈丽梅
%A 玉永雄
%J 植物学报
%D 2010
%I 
%X Stylo (Stylosanthes guianensis) is a forage grass grown worldwide. A cultivar of stylo (‘Reyan No.2’) was shown to be able to exudate citrate and tolerate aluminum (Al). To identify Al-induced genes in stylo, we constructed a forward cDNA library for Reyan No.2 under 300 μmol·L–1 Al stress by suppression subtractive hybridization. Approximately 600 clones with inserts > 300 bp were selected for sequence analysis. In total, 504 expressed sequence tags (ESTs) were obtained: 12.1% were singletons and 61.4% presented as 2–16 copies. The highly repetitive ESTs were 10.5% for cytochrome P450-like TBP protein, with 53 copies; 8.7% for pathogen-inducible trypsin-inhibitor-like protein, with 44 copies; and 7.3% for putative senescence-associated protein, with 37 copies. BLASTX analysis revealed that the 504 ESTs could be assembled into 97 non-redundant sequences, including 46 genes with known function. Of 46 genes with known function, 30 were previously reported to be Al-responsive genes and the other 16 ESTs are novel Al-associated genes. The information provides important clues to facilitate understanding the Al detoxification mechanism in stylo at the molecular level.
%K Al stress
%K cDNA library
%K expressed sequence tag
%K Stylosanthes guianensis&prev_q=Stylosanthes guianensis')" href="#"> Stylosanthes guianensis" target="_blank">Stylosanthes guianensis')" href="#"> Stylosanthes guianensis
%K suppression subtractive hybridization<br>铝胁迫
%K cDNA文库
%K 表达序列标签
%K 柱花草
%K 抑制消减杂交
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=6FA3E63C5DA31FE1F99166BE85158129&yid=140ECF96957D60B2&vid=94E7F66E6C42FA23&iid=B31275AF3241DB2D&sid=DC06EBDBAF4E06D3&eid=2A22E972FD97071B&journal_id=1674-3466&journal_name=植物学报&referenced_num=1&reference_num=0