%0 Journal Article
%T Identification and Genetic Analysis of Two Putative SDD1 Alleles in Arabidopsis<br>拟南芥中两个可能的SDD1新等位基因的鉴定与分析
%A 梁凯
%A 钱平平
%A 程曦
%A 秦倩倩
%A 贾瑞玲
%A 张彦萍
%A 侯岁稳
%J 植物学报
%D 2010
%I 
%X SDD1 is a crucial gene regulating stomatal development and encodes a subtilisin-like serine protease. Screening an ethyl methane sulfonate (EMS)-mutagenized population of Arabidopsis thaliana, we isolated two mutants (e281 and g204) with altered stomatal characteristics. The two mutants showed about a 1.5-fold increase in stomatal density and index and formed a clustered stomata similar to the sdd1-1 mutant, but the internal leaf architecture was not altered. Genetic analysis and sequencing results confirmed that e281 and g204 are two novel SDD1 alleles. The mutation results in amino acid changes in the N domain for the substrate binding site and in one of the S domains for the catalytic triad, respectively. Moreover, morphological and physiological data indicate that mutation of different sites within the SDD1 gene lead to different phenotypes. The antagonism among SDD1 alleles can be a negative effect of the gene transvection.
%K <br>等位基因
%K 拟南芥突变体
%K SDD1
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=B88E2908324323D5A86876800D862DCE&yid=140ECF96957D60B2&vid=94E7F66E6C42FA23&iid=38B194292C032A66&sid=3C6F5C97A07587AE&eid=377D325742940769&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=24