%0 Journal Article
%T Reference Genes for Real-time Fluorescence Quantitative PCR in Camellia sinensis<br>茶树实时荧光定量PCR分析中内参基因的选择
%A Meilian Sun
%A Yunsheng Wang
%A Dongqing Yang
%A Chaoling Wei
%A Liping Gao
%A Tao Xia
%A Yu Shan
%A Yang Luo
%A <br>孙美莲
%A 王云生
%A 杨冬青
%A 韦朝领
%A 高丽萍
%A 夏涛
%A 单育
%A 骆洋
%J 植物学报
%D 2010
%I 
%X The selection of a suitable reference gene is an important prerequisite for successful gene expression analysis by real-time fluorescence quantitative PCR (qPCR). We investigated the expression stability of 4 endogenous candidate genes (18S rRNA, GAPDH, β-actin and α-tubulin) in qPCR experiments in different organs and tissues, including buds, leaves, young roots, stem, petals, seeds, and callus, of the tea plant Camellia sinensis (L.) O. Kuntze. The analysis with GeNorm and NormFinder algorithms revealed that β-actin could be used as a reference gene for organs and tissues and GAPDH for mature leaves and callus.
%K Camellia sinensis&prev_q=Camellia sinensis')" href="#">Camellia sinensis" target="_blank">Camellia sinensis')" href="#">Camellia sinensis
%K real-time fluorescence quantitative PCR
%K reference genes<br>茶树
%K 实时荧光定量PCR
%K 内参基因
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=97AE3D0EBF25E42C466FEF50E492F710&aid=CD92D097874AE5946B6277A6FCE0F628&yid=140ECF96957D60B2&vid=94E7F66E6C42FA23&iid=94C357A881DFC066&sid=57EA20F731155703&eid=D5970ECA7D10A7B1&journal_id=1674-3466&journal_name=植物学报&referenced_num=0&reference_num=0