%0 Journal Article
%T etermination of melamine in biscuits by high performance liquid chromatography
高效液相色谱法检测饼干中的三聚氰胺
%A WEI Jin-mei
%A ZHANG Yong-mei
%A WANG Ji-qing
%A CAO Lu-xing
%A LUO Yu-zhu
%A
魏晋梅
%A 张咏梅
%A 王继卿
%A 曹禄兴
%A 罗玉柱
%J 浙江大学学报(农业与生命科学版)
%D 2010
%I Zhejiang University Press
%X A high performance liquid chromatography method was developed for the determination of melamine in biscuits. The biscuit samples were extracted by 1% trichloroacetic acid solution and 2.2% lead acetate,and melamine was separated on a Hypersil ODS C_18 column (100 mm×4.6 mm,5 μm ). Acetonitrile and sodium 1-octanesulfonate solution(pH 3)(15∶85,V/V) were used as mobile phase at a flow rate of 1.0 mL·min~(-1). The detective wavelength was set as 210 nm by an ultraviolet detector.The temperature of column was 40 ℃. The linear ranges for melamine were 0.2-10 μg·mL~(-1). The average recovery rate of this method varied from 80.5% to 93.5% with the relative standard deviation of 7.7% (n=9) when blank sample was fortified standard working solution of 1,2 and 4 μg·mL~(-1).
%K melamine
%K biscuits
%K high performance liquid chromatography (HPLC)
三聚氰胺
%K 饼干
%K 高效液相色谱法
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=1BBB89357915F04E4170E8DCC18911E9&yid=140ECF96957D60B2&vid=933658645952ED9F&iid=CA4FD0336C81A37A&sid=EDA22B444205D04A&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=12