%0 Journal Article
%T A New Approach for Detection Improvement of the Creutzfeldt-Jakob Disorder through a Specific Surface Chemistry Applied onto Titration Well
%A Caroline Mille
%A Dominique Debarnot
%A Willy Zorzi
%A Benaissa El Moualij
%A Isabelle Quadrio
%A Armand Perret-Liaudet
%A Arnaud Coudreuse
%A Gilbert Legeay
%A Fabienne Poncin-Epaillard
%J Biosensors
%D 2012
%I MDPI AG
%R 10.3390/bios2040433
%X This work illustrates the enhancement of the sensitivity of the ELISA titration for recombinant human and native prion proteins, while reducing other non-specific adsorptions that could increase the background signal and lead to a low sensitivity and false positives. It is achieved thanks to the association of plasma chemistry and coating with different amphiphilic molecules bearing either ionic charges and/or long hydrocarbon chains. The treated support by 3-butenylamine hydrochloride improves the signal detection of recombinant protein, while surface modification with the 3,7-dimethylocta-2,6-dien-1-diamine (geranylamine) enhances the sensitivity of the native protein. Beside the surface chemistry effect, these different results are associated with protein conformation.
%K amphiphilic molecules
%K cold plasma
%K ELISA titration
%K prion protein
%U http://www.mdpi.com/2079-6374/2/4/433