%0 Journal Article %T Effects of different antioxidants on cryopreservation of Jinhua boar sperm
不同抗氧化剂对金华猪精液冷冻保存效果的影响 %A WANG Zheng-guang %A HUA Jian-qing %A CHEN Xiao-feng %A JIN Gen-lu %A YU Song-dong %A
王争光 %A 华坚青 %A 陈晓峰 %A 金根炉 %A 俞颂东 %J 浙江大学学报(农业与生命科学版) %D 2010 %I Zhejiang University Press %X To compare the effects of different antioxidant concentrations of glutathione (GSH), green tea polyphenols (GTPs), vitamin E (V_E) and superoxide dismutase (SOD) on cryopreservation of Jinhua boar sperm, freezing extenders were supplemented with the GSH (0.125, 0.25 and 0.5 mmol·L~(-1)), GTPs (10, 15 and 20 mmol·L~(-1)), V_E (1, 2 and 4 mg·mL~(-1)) and SOD (1000, 2000 and 4000 IU), respectively. The boar sperms were collected and frozen with extenders containing above antioxidants. Sperm motility after 4 ℃cooling, sperm motility, membrane integrity and acrosome integrity after thawing were examined in four treatment groups. The results show that sperm motility after 4 ℃cooling, sperm motility and membrane integrity after thawing were increased significantly by supplemented boar freezing extenders with 15 mmol·L~(-1) GTPs, 2 mg·mL~(-1) V_E or 4000 IU SOD, respectively (P< 0.05). %K antioxidants %K sperm %K Jinhua boar %K cryoprotective agents
抗氧化剂 %K 精液 %K 金华猪 %K 冷冻保护剂 %U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=A5B02613695222F0BFB95AAF40567687&yid=140ECF96957D60B2&vid=933658645952ED9F&iid=CA4FD0336C81A37A&sid=DBF54A8E2A721A6D&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=12