%0 Journal Article
%T Response surface optimization of medium components for producing cellulase by Cladosporium cladosporioides F4-1<br>枝状枝孢菌(Cladosporium cladosporioides)发酵产酶培养基的响应面法优化
%A HAN Li-rong
%A WANG Yong-hong
%A HE Jun
%A AN Feng-qiu
%A ZHANG Xing
%A <br>韩立荣
%A 王永宏
%A 何军
%A 安凤秋
%A 张兴
%J 浙江大学学报(农业与生命科学版)
%D 2010
%I Zhejiang University Press
%X The medium of liquid fermentation for producing CMCase (carboxymethyl cellulase) by Cladosporium cladosporioides strain F4-1 was optimized using response surface methodology (RSM). The optimum carbon, nitrogen and inorganic salts were obtained with one-factor-at-a-time. A central composite design and RSM were applied to determine the optimal concentration of each component above. The results show that the optimal medium was as follows:wheat straw 23.18 g·L～(-1),wheat bran 30.00 g·L～(-1), yeast extract 13.62 g·L～(-1),KH_2PO_4 4.60 g·L～(-1),NaCl 4.60 g·L～(-1),MgSO_4·7H_2O 0.46 g·L～(-1) . Under this condition, the CMCase activity reached 2.80 U·mL～(-1), which increased 2.18 times than before optimization.
%K Cladosporium cladosporioides
%K cellulase
%K response surface methodology
%K liquid fermentation<br>枝状枝孢菌
%K 纤维素酶
%K 响应面法
%K 液体发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=875CF396D62F28F1F58E448FEE16C9EB&yid=140ECF96957D60B2&vid=933658645952ED9F&iid=CA4FD0336C81A37A&sid=014B591DF029732F&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=0&reference_num=17