%0 Journal Article
%T A real-time PCR method for rapid detection of Gymnodinium sanguineum<br>红色裸甲藻实时定量PCR快速检测方法的建立(英文)
%A HE Shan-ying
%A YU Zhi-gang
%A <br>何闪英
%A 于志刚
%J 浙江大学学报(农业与生命科学版)
%D 2009
%I Zhejiang University Press
%X For detecting the harmful algal bloom(HAB)species sensitively and rapidly,Gymnodinium sanguineum was taken as the object,and the rapid detection method-RFQ-PCR(real-time fluorescent quantitative polymerase chain reaction)technique was applied to the HAB species detection.Firstly,gene specific primers and a TaqMan DNA probe were designed based on the sequence of 18S rDNA cloned from G.sanguineum.Then,using the primers and probe combination.a RFQ-PCR method was developed for quantitative detection of G.sanguineum.a harmful algae bloom species.The RFQ-PCR assay data showed good agreement with traditional microscope counting methods,suggesting that RFQPCR may be a useful method for detecting G.sanguineum.The detection threshold tested was much lower than that of G.sanguineum HAB.so this technique will be useful for forecasting and preventing G.sanguineum HAB.
%K 18S
%K rDNA
%K Gymnodinium sanguineum
%K fluorescent quantitative PCR
%K 18S rDNA
%K harmful algal bloom<br>红色裸甲藻
%K 荧光定量PCR
%K 赤潮
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=466C05D88593309B3EB5CF34361E6426&aid=B6EB4D6AF438EE7C098AAF783473EAB8&yid=DE12191FBD62783C&vid=6209D9E8050195F5&iid=0B39A22176CE99FB&sid=EFD65B51496FB200&eid=6FBD78E3BAB60869&journal_id=1008-9209&journal_name=浙江大学学报(农业与生命科学版)&referenced_num=2&reference_num=45