%0 Journal Article
%T A High-speed Automatic System for Measuring the Mechanical Properties of Cells<br>高速自动化细胞机械特性测量系统
%A WANG Zhi-Bo
%A LIU Lian-Qing
%A WANG Yue-Chao
%A XI Ning
%A DONG Zai-Li
%A HUANG Shu-Tao
%A <br>王智博
%A 刘连庆
%A 王越超
%A 席宁
%A 董再励
%A 黄树涛
%J 自动化学报
%D 2012
%I 
%X As a novel effective label-free biomarker, the mechanical properties of cells have become increasingly important. However, the current methods of mapping the cellular mechanical properties are mostly carried out manually and this results in that the measurements are time consuming, which can not meet the demand of testing a large quantity of cell samples for biological statistical analysis. In this paper, a high-speed automatic system for measuring the mechanical properties of cells based on atomic force microscopy (AFM) is proposed. In this system, cells are recognized using an image processing method and the relative position of the cell with respect to AFM tip is accurately calibrated by the local scan method, i.e., the mechanical properties of cells can be measured sequentially without performing the step of AFM imaging. Besides, with the automation implementation, the high-throughput measurement of cellular mechanical properties can be performed rapidly. Experimental results show that the measurement efficiency of our system is 27 times faster than that of the traditional method.
%K Automation
%K mechanical properties of cells
%K atomic force microscopy (AFM)
%K local scan<br>自动化
%K 细胞机械特性
%K 原子力显微镜
%K 局部扫描
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=8240383F08CE46C8B05036380D75B607&jid=E76622685B64B2AA896A7F777B64EB3A&aid=21CB0722CA48644FA5BFEA919CE56701&yid=99E9153A83D4CB11&vid=16D8618C6164A3ED&iid=F3090AE9B60B7ED1&sid=71706E59E547123D&eid=E521CC33DC22B84E&journal_id=0254-4156&journal_name=自动化学报&referenced_num=0&reference_num=17