%0 Journal Article
%T The expression of antibacterial peptide CM4 in Escherichia coli fused with human soluble B lymphocyte stimulator activing factor<br>抗菌肽CM4与人可溶性B淋巴细胞因子hsBAFF在大肠杆菌中的融合表达
%A LIU Hai-feng
%A WANG Xiao-hua
%A LIU Ping
%A ZHANG Shuang-quan
%A <br>刘海峰
%A 王小花
%A 刘平
%A 张双全
%J 中国生物工程杂志
%D 2008
%I 
%X To explore the expression and function of antibacterial peptide CM4, the expression and biological activity of recombinant fusion protein CM4-hsBAFF were studied. The human soluble B lymphocyte stimulator activing factor (hsBAFF) gene was fused to the sequence encoding CM4 to construct an expression vector pET28a (+)/CM4-hsBAFF. The recombinant protein was high expression of soluble recombinant protein in Escherichia coli cells, and existed in the supernatant after sonication. Recombinant fusion protein which was purified through size-exclusion chromatography was identified by SDS-PAGE and Western blot analysis. SDS-PAGE and Western blot indicated that recombinant protein was secreted as a protein of around 22.0 KDa and can be specially recognized anti-hsBAFF antibody. The recombinant protein can be expressed and displays antimicrobial activity.
%K antibacterial peptide CM4
%K hsBAFF
%K expression
%K purification<br>抗菌肽CM4
%K 人可溶性B淋巴细胞因子
%K 表达
%K 纯化
%K 抗菌肽
%K 人可溶性
%K 淋巴
%K 细胞因子
%K 大肠杆菌
%K 融合表达
%K Factor
%K Stimulator
%K B
%K Lymphocyte
%K Human
%K Escherichia
%K coli
%K Peptide
%K Antibacterial
%K 抑菌
%K 方法
%K 基因工程
%K 特异性反应
%K 发生
%K 抗体
%K 鼠抗人
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=477524B098D12A5AF15DFC833CDDCEB9&yid=67289AFF6305E306&vid=D3E34374A0D77D7F&iid=38B194292C032A66&sid=9971A5E270697F23&eid=42425781F0B1C26E&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=10