%0 Journal Article
%T Activity study of specific bFGF-binding phage<br>bFGF特异性结合噬菌体的活性研究
%A YAN Qiu-xia
%A WU Xiao-ping
%A HUANG Hui-xian
%A NIE Chang-jun
%A NIE Yan-fang
%A KE Shi
%A XIAO Jian
%A LI Xiao-kun
%A <br>颜秋霞
%A 吴晓萍
%A 黄慧贤
%A 聂昌君
%A 聂燕芳
%A 柯实
%A 肖健
%A 李校堃
%J 中国生物工程杂志
%D 2009
%I 
%X To study the activity of a specific bFGF-binding phage selected from a phage display heptapeptide library. ELISA was applied to assess the bFGF-binding curve of the positive phage clone obtained from 3 rounds screening. The competitive binding activity of the bFGF-binding phage was examined by competitive inhibition assay. The effect of the bFGF-binding phage on the proliferation of Balb/c 3T3 cells induced by bFGF was investigated by MTT method. The positive phage selected from the phage library specifically band to bFGF in a dose-dependent manner, and its binding with the immobilized bFGF was competitively inhibited by free bFGF. The isolated phage could also inhibit the proliferation of Balb/c 3T3 cells induced by bFGF. The bFGF-binding phage could inhibit bFGF activity, which would offer the basis for further developing the anti-tumor short peptide targeting bFGF.
%K bFGF<br>噬菌体展示肽库
%K 碱性成纤维细胞生长因子
%K 结合噬菌体
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=951380788B355DEA7D75520FA3B199C9&aid=730F7B128198D6E0E92A5D29370B4CF0&yid=DE12191FBD62783C&vid=771469D9D58C34FF&iid=CA4FD0336C81A37A&sid=EA389574707BDED3&eid=96C778EE049EE47D&journal_id=1671-8135&journal_name=中国生物工程杂志&referenced_num=0&reference_num=10