%0 Journal Article
%T Cloning and Tissue Expression Analysis of Creatine Kinase (M-CK) cDNA from the Mandarin Fish, Siniperca chuatsi<br>鳜肌酸激酶M-CK cDNA的克隆与组织表达分析
%A ZHANG Min
%A ZHAO Jin-Liang
%A DENG Yan-Fei
%A <br>张敏
%A 赵金良
%A 邓燕飞
%J 动物学研究
%D 2010
%I Chinese Academy of Sciences
%X The creatine kinase(CK) cDNA from the mandarin fish Siniperca chuatsi was cloned by RT-PCR and rapid amplification of cDNA ends (RACE) methods.The structural characteristics and phylogeny of this gene were analyzed.Sequence analysis revealed a 1586 bp cDNA sequence containing 92 bp 5'-untranslated region,348 bp 3'-untranslated region and 1146 bp open reading frame (ORF),which encoded 381 amino acids.Conserved sequence blocks of vertebrate CKs and diagnostic boxes for the muscle CK(M-CK) isozyme were identif...
%K Siniperca chuatsi
%K Creatine kinase
%K Rapid amplification of cDNA ends
%K Tissue expression<br>鳜
%K 肌酸激酶
%K cDNA末端快速扩增
%K 组织表达
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=4F31E3EEE9950DC66F45EBC945ABDD46&yid=140ECF96957D60B2&vid=4AD960B5AD2D111A&iid=CA4FD0336C81A37A&sid=E44E40A2398D4F2A&eid=06EA2770E96C5402&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=18