%0 Journal Article
%T Rhesus monkey embryonic stem cells differentiation, proliferation and allotransplantation<br>猕猴神经干细胞的诱导分化、干性维持及同种脑内移植
%A Dong Jin-Run
%A Guo Li-Yun
%A Qu Jia-Gui
%A Qi Ren-Li
%A Wang Wen-Chao
%A Xiao Chun-Jie
%A Wang Zheng-Bo
%A <br>Dong JR
%A Guo LY
%A Qu JG
%A Qi RL
%A Wang WC
%A Xiao CJ
%A Wang ZB
%J 动物学研究
%D 2012
%I Chinese Academy of Sciences
%X To investigate the characteristics of rhesus monkey embryonic stem cells and to promote their clinical application, the differentiation and proliferation of rosettes neural stem cells from GFP marked rhesus monkey embryonic stem cells were studied The results showed that: 1) A stable and high-efficient neural differentiation system was established. More than 95% of the embryonic stem cells were differentiated into neural stem cells on the 12th days of differentiation; 2) the rosettes neural stem cells differentiated from the rhesus monkey embryonic stem cells could maintain their rosettes-shape by proliferating with bFGF/EGF; 3) the neural stem cells could differentiate into neurons after transplanted into the rhesus monkey brain. In conclusion, the rosettes neural stem cells differentiated from rhesus monkey embryonic stem cells could maintain their characteristics after proliferation with bFGF/EGF and they could survive and differentiate into neurons after transplanted into the rhesus monkey brain, which strongly supports the clinical application of neural stem cells in the future.
%K Rhesus monkey
%K Neural stem cells
%K Rosettes
%K Basic fibroblast growth factor
%K Epidermal growth factor<br>猕猴
%K 神经干细胞
%K 玫瑰花环结构
%K 碱性成纤维细胞生长因子
%K 表皮生长因子
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=90BA3D13E7F3BC869AC96FB3DA594E3FE34FBF7B8BC0E591&jid=7C502F1A6798ACE1B8C57E291C48C7F1&aid=1D36885FD7A8294B564218E5B378B181&yid=99E9153A83D4CB11&vid=27746BCEEE58E9DC&iid=CA4FD0336C81A37A&sid=BE33CC7147FEFCA4&eid=B6DA1AC076E37400&journal_id=0254-5853&journal_name=动物学研究&referenced_num=0&reference_num=64