%0 Journal Article
%T Optimization of γ-Polyglutamic Acid Production by Bacillus licheniformis P-104<br>地衣芽孢杆菌P-104发酵生产γ-聚谷氨酸条件优化
%A ZHANG Ye-wei
%A WEI Xue-tuan
%A HU Zhong-bo
%A LUO Ming-fang
%A XU Lin
%A LIU Hui-zhou
%A <br>张业伟
%A 魏雪团
%A 胡中波
%A 罗明芳
%A 徐林
%A 刘会洲
%J 过程工程学报
%D 2012
%I 
%X The fermentation conditions for γ-polyglutamic acid (γ-PGA) production with Bacillus licheniformis P-104 were optimized in flasks, and the mesoscale batch fermentation was performed in a 6.6 L fermentor. The results showed that high yield of g-PGA with ultra high molecular weight (over 2500 kDa) was obtained under the optimal conditions (g/L): glucose 80, sodium glutamate 70, sodium citrate 10, (NH4)2SO4 10, MnSO4 0.15, MgSO4 0.8, K2HPO4 0.6, NaNO3 4, inoculation time 8 h, inoculum amount 3%(ψ), initial pH value 7.5, 37℃, and 180 r/min. In the flask fermentation, the maximum γ-PGA yield reached 44.7 g/L for 24 h, and the highest specific productivity was 1.49 g/(L·h), being twice of the ultra high molecular weight γ-PGA reported previously. In a 6.6 L fermentor, 32 g/L γ-PGA was obtained for 33 h with a specific productivity of 0.97 g/(L·h).
%K Bacillus licheniformis
%K γ-polyglutamic acid
%K optimization
%K batch fermentation<br>地衣芽孢杆菌
%K γ-聚谷氨酸
%K 优化
%K 批式发酵
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=5B3AB970F71A803DEACDC0559115BFCF0A068CD97DD29835&cid=3FCF8B1A330466D5&jid=B9EE12934D19905403D996AE65CEEEED&aid=B39D47F72CB13A768BFDEB38D30157FF&yid=99E9153A83D4CB11&vid=59906B3B2830C2C5&iid=0B39A22176CE99FB&sid=334C61CAF4C8EF4E&eid=8BB50A069C48D50B&journal_id=1009-606X&journal_name=过程工程学报&referenced_num=0&reference_num=0