%0 Journal Article
%T The Primary Study on Construction of RNAi Expression Vector of sbe2a Gene and Maize Transformation<br>玉米sbe2a基因RNAi载体的构建及转化玉米的初步研究
%A GUAN Shu-yan
%A CHU Hai-jiao
%A LIU Hui-jing
%A LIU Guang-na
%A LIU Xue-zhi
%A WANG Pi-wu
%A <br>关淑艳
%A 楚海娇
%A 刘慧婧
%A 刘广娜
%A 刘学志
%A 王丕武
%J 吉林农业大学学报
%D 2011
%I 
%X cDNA segment of corn starch branching enzymes gene sbe2a was cloned by PCR technique and inserted into pMD18-T vector.The recombinant clone was detected by PCR and the sequence and fragments of restriction enzymes were analyzed.The results showed that the length of the clone sequence was 562 bp.The sense and anti-sense fragments of clone sequence were inserted into downstream of 35S promoter of pCAMBIA1301 to construct over-expression RNAi vector.And then it was transformed into corn inbred lines TIE 7922 b...
%K maize
%K starch branching enzyme gene sbe2a
%K RNAi
%K genetic transformation<br>玉米
%K 淀粉分支酶基因sbe2a
%K RNAi
%K 遗传转化
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=74C612B26EF38BF0D4C997E3F9E167A1&aid=5704D2E38E2B920F234C02AD933C5369&yid=9377ED8094509821&vid=27746BCEEE58E9DC&iid=0B39A22176CE99FB&sid=9D453329DCCABB94&eid=5BC9492E1D772407&journal_id=1000-5684&journal_name=吉林农业大学学报&referenced_num=0&reference_num=15