%0 Journal Article
%T Rapid Mini-Preparation of Plasmid DNA for Eliminating False Positive Clones<br>小提质粒快速排除假阳性克隆的新方法
%A REN Da-yong
%A LI Chang
%A QIN Yan-qing
%A DU Shou-wen
%A ZHU Qing-li
%A JIN Ning-yi
%A <br>任大勇
%A 李昌
%A 秦艳青
%A 杜寿文
%A 诸晴丽
%A 金宁一
%J 吉林农业大学学报
%D 2011
%I 
%X A rapid method was developed to greatly simplify the procedures of plasmid DNA purification within about 15 min and greatly reduce the experiment cost compared with traditional method. Seven recombinants were constructed and identified by PCR in order to confirm reliability of the new method.The results indicated that this new method was simple and inexpensive and suitable for gram-negative bacterium.It can be used for rapidly eliminating false positive clones.
%K plasmid
%K mini-preparation
%K false postwe clone<br>质粒
%K 小量制备
%K 假阳性克隆
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=03F54A49DE00578AA0E5DDF5BC021AA7&cid=298920A27C9BAA22346FCA384240FAA4&jid=74C612B26EF38BF0D4C997E3F9E167A1&aid=5704D2E38E2B920FAF5A2AA4A0C6B031&yid=9377ED8094509821&vid=27746BCEEE58E9DC&iid=0B39A22176CE99FB&sid=D46BA3D3D4B3C585&eid=847B14427F4BF76A&journal_id=1000-5684&journal_name=吉林农业大学学报&referenced_num=0&reference_num=9