%0 Journal Article
%T microRNA-16 expression in HeLa cells is upregulated in cell-cycle and drug dependent manner<br>
%A Jie Li
%A JiaRui Wu
%A <br>
%J 科学通报(英文版)
%D 2011
%I 
%X microRNAs are single-stranded, non-coding RNAs that regulate gene expression. The microRNA-16 family has been reported to be involved in cell-cycle regulation, which could also downregulate expression of multiple pro-proliferation genes. The present results demonstrated that miR-16 expression in HeLa cells increased when the cells were arrested during S-phase under methyl methanesulfate (MMS) treatment. This further resulted in downregulation of a target protein CDC25A, whereas miR-16 expression did not increase when HeLa cells were arrested during the MMS-treated G0/G1 or G2/M phase. Furthermore, when HeLa cells were arrested during S-phase with hydroxyurea treatment, miR-16 expression did not increase. These results suggest that expression levels of microRNAs in mammalian cells are delicately regulated under variable cellular conditions.
%K microRNA
%K cell cycle
%K DNA damage
%K methyl methanesulfate
%K HeLa cells<br>
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=96E6E851B5104576C2DD9FC1FBCB69EF&jid=DD6615BC9D2CFCE0B6F945E8D5314523&aid=0548E7C9AB7F8CD246B9546B04DB5C05&yid=9377ED8094509821&vid=014B591DF029732F&iid=CA4FD0336C81A37A&sid=339D79302DF62549&eid=16D8618C6164A3ED&journal_id=1001-6538&journal_name=科学通报(英文版)&referenced_num=0&reference_num=19