%0 Journal Article
%T Microplate chemiluminescence immunoassay for determination of triiodothyronine in human serum<br>微孔板化学发光免疫分析法检测人血清中三碘甲状腺原氨酸
%A WANG ALian
%A XIAO Qin
%A YING XiTang
%A CHEN GuoNan
%A LIN JinMing College of Chemistry
%A Chemical Engineering
%A Fuzhou University
%A Fuzhou
%A <br>汪阿恋
%A 肖勤
%A 应希堂
%A 陈国南
%A 林金明
%J 科学通报
%D 2010
%I 
%X A microplate chemiluminescence immunoassay(CLIA) was developed for the clinical determination of Triiodothyronine(T3) in human serum,and the luminal-hydrogen peroxide was used as chemiluminescence system catalyzed by horseradish peroxidase(HRP).Several physicochemical parameters such as the dilution ratios of coated antibody and HRP-T3 conjugate was chose,the incubation time and detection time were optimized.Methodology evaluation was done:the detection limit was 0.07 ng/mL;intra-assay and inter-assay coeff...
%K triiodothyronine
%K chemiluminescence immunoassay
%K horseradish peroxidase<br>三碘甲状腺原氨酸
%K 化学发光免疫分析
%K 辣根过氧化物酶
%U http://www.alljournals.cn/get_abstract_url.aspx?pcid=01BA20E8BA813E1908F3698710BBFEFEE816345F465FEBA5&cid=7C7E63796F062382A606A3A9833B8C05&jid=B40D4BA57FF46E45205A09B4DC283152&aid=FBFFCD85039E26FF251B1552A18A6F29&yid=140ECF96957D60B2&vid=E514EE58E0E50ECF&iid=CA4FD0336C81A37A&sid=A04140E723CB732E&eid=C5154311167311FE&journal_id=0023-074X&journal_name=科学通报&referenced_num=0&reference_num=26