%0 Journal Article
%T PCV2-DNA in formalin-fixed and paraffin embedded lymph nodes of wild boar (Sus scrofa ssp. scrofa): one sampling approach for two laboratory techniques
%A Federico Morandi
%A Serena Panarese
%A Ranieri Verin
%A Fabio Ostanello
%A Cinzia Benazzi
%A Giuseppe Sarli
%J Acta Veterinaria Scandinavica
%D 2012
%I BioMed Central
%R 10.1186/1751-0147-54-17
%X Since 1998, Porcine Circovirus type 2 (PCV2) has been recognized to play an important role in postweaning multisystemic wasting syndrome (PMWS), as well as in many other pathologies in pig [1] defined as PCVD (PCV2 Diseases), causing huge economic losses to swine husbandry in all affected countries. The host spectrum is limited to the genus Sus [1] and numerous studies report in wild boar both PCV2 infection and associated diseases [2-5]. The wide spread of the infection and the absence of a close correlation between this and the pathological description made in situ tests (immunohistochemistry-IHC and in situ hybridization-ISH) the gold standard for the diagnosis of PCVD [1], whereby the causative agent is highlighted in the lesion. Despite this, it is also true that the greater sensitivity of PCR based methods [6] can provide more accurate information in assessing the infection prevalence in wild boar [3,7,8]. In many cases, paraffin embedded material is available as well as frozen serum samples [9,10].In the light of that, some studies have developed sophisticated techniques to extract viral nucleic acid, from formalin-fixed and paraffin-embedded (FFPE) specimens, sufficiently preserved to be submitted for biomolecular investigations [10,11]. The objectives of the present study were: 1) to carry out PCV2-DNA extraction and a subsequent investigation on FFPE with PCR in wild boar; 2) to compare the PCR results with those obtained on the same samples by IHC.In a previous study [2], 148 superficial inguinal lymph nodes, from as many wild boar shot in the Bologna Province (44กใ00'N, 11กใ00'E) and in the Colli Euganei Regional Park (45กใ14'N, 11กใ45'E), were examined with an IHC technique. Within the total amount, 72 lymph nodes were selected according to the following criteria: PCV2-IHC strongly positive lymph nodes with PMWS histological lesions (4 samples; outcome 1); weak to mild PCV2-IHC positivity without PMWS histological lesions (6 samples; outcome 2); randomly ch
%U http://www.actavetscand.com/content/54/1/17