%0 Journal Article
%T Enhanced effects of cigarette smoke extract on inflammatory cytokine expression in IL-1汕-activated human mast cells were inhibited by Baicalein via regulation of the NF-百B pathway
%A David S Chi
%A Ta-Chang Lin
%A Kenton Hall
%A Tuanzhu Ha
%A Chuanfu Li
%A Zong Wu
%A Thomas Soike
%A Guha Krishnaswamy
%J Clinical and Molecular Allergy
%D 2012
%I BioMed Central
%R 10.1186/1476-7961-10-3
%X Main-stream (Ms) and Side-stream (Ss) cigarette smoke were collected onto fiber filters and extracted in RPMI-1640 medium. Two ml of HMC-1 at 1 ℅ 106 cells/mL were cultured with CSE in the presence or absence of IL-1汕 (10 ng/mL) for 24 hrs. A group of HMC-1 cells stimulated with both IL-1汕 (10 ng/ml) and CSE was also treated with BAI. The expression of IL-6 and IL-8 was assessed by ELISA and RT-PCR. NF-百B activation was measured by electrophoretic mobility shift assay (EMSA) and I百B汐 degradation by Western blot.Both Ms and Ss CSE significantly increased IL-6 and IL-8 production (p < 0.001) in IL-1汕-activated HMC-1. CSE increased NF-百B activation and decreased cytoplasmic I百B汐 proteins in IL-1汕-activated HMC-1. BAI (1.8 to 30 米M) significantly inhibited production of IL-6 and IL-8 in a dose-dependent manner in IL-1汕-activated HMC-1 with the optimal inhibition concentration at 30 米M, which also significantly inhibited the enhancing effect of CSE on IL-6 and IL-8 production in IL-1汕-activated HMC-1. BAI inhibited NF-百B activation and increased cytoplasmic I百B汐 proteins in CSE-treated and IL-1汕-activated HMC-1.Our results showed that CSE significantly increased inflammatory cytokines IL-6 and IL-8 production in IL-1汕-activated HMC-1. It may partially explain why cigarette smoke contributes to lung and cardiovascular diseases. BAI inhibited the production of inflammatory cytokines through inhibition of NF-百B activation and I百B汐 phosphorylation and degradation. This inhibitory effect of BAI on the expression of inflammatory cytokines induced by CSE suggests its usefulness in the development of novel anti-inflammatory therapies.Human mast cells, which are associated with allergies, asthma, and atherosclerosis, are multifunctional cells capable of inflammatory responses producing and secreting a wide variety of lipid mediators, histamine, cytokines, and chemokines [1,2]. Mast cells have been implicated in acute and chronic inflammatory responses and in many diseases charact
%K Mast cell
%K cigarette smoking
%K Baicalein
%K IL-6
%K IL-8
%K NF-百B activation
%K I百B汐 phosphorylation and degradation
%U http://www.clinicalmolecularallergy.com/content/10/1/3