%0 Journal Article
%T Interactions Between Estrogen- and Ah-Receptor Signalling Pathways in Primary Culture of Salmon Hepatocytes Exposed to Nonylphenol and 3,3',4,4'-Tetrachlorobiphenyl (Congener 77)
%A Anne S Mortensen
%A Augustine Arukwe
%J Comparative Hepatology
%D 2007
%I BioMed Central
%R 10.1186/1476-5926-6-2
%X Our data showed a reciprocal inhibitory interaction between NP and PCB-77. PCB-77 produced anti-NP-mediated effect by decreasing the mRNA expression of ER-responsive genes. NP produced anti-AhR mediated effect or as inhibitor of AhR¦Á, AhRR, ARNT, CYP1A1 and UDPGT expression. A novel aspect of the present study is that low (0.001 ¦ÌM) and medium (0.01 ¦ÌM) PCB-77 concentrations increased ER¦Á mRNA expression above control and NP exposed levels, and at 12 h post-exposure, PCB-77 exposure alone produced significant elevation of ER¦Á, ER¦Â and Zr-protein expressions above control levels.The findings in the present study demonstrate a complex mode of ER-AhR interactions that were dependent on time of exposure and concentration of individual chemicals (NP and PCB-77). This complex mode of interaction is further supported by the effect of PCB-77 on ER¦Á and ER¦Â (shown as increase in transcription) with no concurrent activation of Vtg (but Zr-protein) response. These complex interactions between two different classes of ligand-activated receptors provide novel mechanistic insights on signalling pathways. Therefore, the degree of simultaneous interactions between the ER and AhR gene transcripts demonstrated in this study supports the concept of cross-talk between these signalling pathways.Halogenated organic contaminants such as 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), polychlorinated biphenyls (PCBs), and polycyclic aromatic hydrocarbons (PAHs) are notorious environmental pollutants that cause acute and chronic toxicity [1]. Several of these compounds including planar PCBs, exert their biological effects through the aryl hydrocarbon receptor (AhR or Ah-receptor). The AhR is a ligand activated transcription factor that regulates the activation of several genes encoding phase I and II biotransformation enzymes [2]. The AhR belongs to the family of basic helix-loop-helix (BHLH)/Per-ARNT-Sim (PAS) proteins that are characterized by two conserved domains, the N-terminal bHLH and th
%U http://www.comparative-hepatology.com/content/6/1/2