%0 Journal Article
%T Characterization of the seminal plasma proteome in men with prostatitis by mass spectrometry
%A Daniel Kagedan
%A Irene Lecker
%A Ihor Batruch
%A Christopher Smith
%A Ilia Kaploun
%A Kirk Lo
%A Ethan Grober
%A Eleftherios P Diamandis
%A Keith A Jarvi
%J Clinical Proteomics
%D 2012
%I BioMed Central
%R 10.1186/1559-0275-9-2
%X Using mass spectrometry, we identified 1708 proteins in the pooled seminal plasma of 5 prostatitis patients. Comparing this list to a previously published list of seminal plasma proteins in the pooled seminal plasma of 5 healthy, fertile controls yielded 1464 proteins in common, 413 found only in the control group, and 254 found only in the prostatitis group. Applying a set of criteria to this dataset, we generated a high-confidence list of 59 candidate prostatitis biomarkers, 33 of which were significantly increased in prostatitis as compared to control, and 26 of which were decreased. The candidates were analyzed using Gene Ontology and Ingenuity Pathway analysis to delineate their subcellular localizations and functions.Thus, in this study, we identified 59 putative biomarkers in seminal plasma that need further validation for diagnosis and monitoring of prostatitis.Prostatitis is a very common condition, with symptoms affecting approximately 10% of all men [1]. Asymptomatic prostatitis likely affects many more men. Prostatitis is the most common urologic diagnosis in males younger than 50 years, and the third most common diagnosis in males older than 50. Annually, prostatitis accounts for approximately 2 million outpatient visits to urology practices in the United States alone [2].The symptoms associated with prostatitis are extremely variable in location, severity, duration and type. The symptom complex includes increased urinary frequency, urgency, and dysuria, as well as pain in the pelvic region, penis and urethra. The symptoms range from mild to disabling and may be of limited duration, or chronic, with episodic worsening. With such a variable phenotype, it is not surprising that prostatitis is a challenge to diagnose and treat.Prostatitis may also be asymptomatic, with an adverse effect on fertility. This condition has been associated with male infertility through a mechanism involving inflammatory cytokines and their negative impact on fertility [3,4].Cur
%K prostatitis
%K seminal plasma
%K inflammation
%K biomarkers
%K mass spectrometry
%U http://www.clinicalproteomicsjournal.com/content/9/1/2