%0 Journal Article
%T Unique and differential protein signatures within the mononuclear cells of HIV-1 and HCV mono-infected and co-infected patients
%A Nawal Boukli
%A Vivekananda Shetty
%A Luis Cubano
%A Martha Ricaurte
%A Jordana Coelho-dos-Reis
%A Zacharie Nickens
%A Punit Shah
%A Andrew H. Talal
%A Ramila Philip
%A pooja Jain
%J Clinical Proteomics
%D 2012
%I BioMed Central
%R 10.1186/1559-0275-9-11
%X In this study, we investigated the proteome profiles of peripheral blood mononuclear cells from HIV-1 mono-, HCV mono-, and HIV-1/HCV co-infected patients. The results of high-resolution 2D gel electrophoresis and PD quest software quantitative analysis revealed that several proteins were differentially expressed in HIV-1, HCV, and HIV-1/HCV co-infection. Liquid chromatography-mass spectrometry and Mascot database matching (LC-MS/MS analysis) successfully identified 29 unique and differentially expressed proteins. These included cytoskeletal proteins (tropomyosin, gelsolin, DYPLSL3, DYPLSL4 and profilin-1), chaperones and co-chaperones (HSP90-beta and stress-induced phosphoprotein), metabolic and pre-apoptotic proteins (guanosine triphosphate [GTP]-binding nuclear protein Ran, the detoxifying enzyme glutathione S-transferase (GST) and Rho GDP-dissociation inhibitor (Rho-GDI), proteins involved in cell prosurvival mechanism, and those involved in matrix synthesis (collagen binding protein 2 [CBP2]). The six most significant and relevant proteins were further validated in a group of mono- and co-infected patients (n = 20) at the transcriptional levels. Conclusions: The specific pro and anti- apoptotic protein signatures revealed in this study could facilitate the understanding of apoptotic and protective immune-mediated mechanisms underlying HIV-1 and HCV co-infection and their implications on liver disease progression in co-infected patients.
%U http://www.clinicalproteomicsjournal.com/content/9/1/11/abstract