%0 Journal Article
%T Pervasive and dynamic protein binding sites of the mRNA transcriptome in Saccharomyces cerevisiae
%A Mallory A Freeberg
%A Ting Han
%A James J Moresco
%A Andy Kong
%A Yu-Cheng Yang
%A Zhi JOHN Lu
%A John R Yates
%A John K Kim
%J Genome Biology
%D 2013
%I BioMed Central
%R 10.1186/gb-2013-14-2-r13
%X Here, we describe the gPAR-CLIP (global photoactivatable-ribonucleoside-enhanced crosslinking and immunopurification) approach for capturing regions of the untranslated, polyadenylated transcriptome bound by RNA-binding proteins (RBPs) in budding yeast. We report over 13,000 RBP crosslinking sites in untranslated regions (UTR) covering 72% of protein-coding transcripts encoded in the genome, confirming 3' UTRs as major sites for RBP interaction. Comparative genomic analyses reveal that RBP crosslinking sites are highly conserved, and RNA folding predictions indicate that secondary structural elements are constrained by protein binding and may serve as generalizable modes of RNA recognition. Finally, 38% of 3' UTR crosslinking sites show changes in RBP occupancy upon glucose or nitrogen deprivation, with major impacts on metabolic pathways as well as mitochondrial and ribosomal gene expression.Our study offers an unprecedented view of the pervasiveness and dynamics of protein-RNA interactions in vivo.
%U http://genomebiology.com/2013/14/2/R13/abstract