%0 Journal Article
%T Differential effects of diazepam and MPEP on habituation and neuro-behavioural processes in inbred mice
%A Amber R Salomons
%A Nathaly Espitia Pinzon
%A Hetty Boleij
%A Susanne Kirchhoff
%A Saskia S Arndt
%A Rebecca E Nordquist
%A Lothar Lindemann
%A Georg Jaeschke
%A Will Spooren
%A Frauke Ohl
%J Behavioral and Brain Functions
%D 2012
%I BioMed Central
%R 10.1186/1744-9081-8-30
%X To test this hypothesis and extend our knowledge on the behavioural profile of 129P3 mice, the effects of the anxiolyticdiazepam (1, 3 and 5£¿mg/kg) and the putative anxiolytic metabotropic glutamate receptor 5 (mGlu5R) antagonist 2-methyl-6-(phenylethynyl)pyridine (MPEP, 3, 10 and 30£¿mg/kg) treatment on within-trial (intrasession) habituation, object recognition (diazepam: 1£¿mg/kg; MPEP 10£¿mg/kg) and on the central-nervous expression of the immediate early gene c-Fos (diazepam: 1£¿mg/kg; MPEP 10£¿mg/kg) were investigated.Behavioural findings validated the initially high, but habituating phenotype of BALB/c mice, while 129P3 mice were characterized by impaired intrasession habituation. Diazepam had an anxiolytic effect in BALB/c mice, while in higher doses caused behavioural inactivity in 129P3 mice. MPEP revealed almost no anxiolytic effects on behaviour in both strains, but reduced stress-induced corticosterone responses only in 129P3 mice. These results were complemented by reduced expression of c-Fos after MPEP treatment in brain areas related to emotional processes, and increased c-Fos expression in higher integrating brain areas such as the prelimbic cortex compared to vehicle-treated 129P3 mice.These results suggest that the strain differences observed in (non)adaptive anxiety behaviour are at least in part mediated by differences in gamma-aminobutyric acid- A and mGluR5 mediated transmission.
%K Anxiety
%K Behavior
%K Benzodiazepines
%K C-Fos
%K Cortisosterone
%K mGluR5 antagonist
%U http://www.behavioralandbrainfunctions.com/content/8/1/30/abstract