%0 Journal Article
%T Rapid paracellular transmigration of Campylobacter jejuni across polarized epithelial cells without affecting TER: role of proteolytic-active HtrA cleaving E-cadherin but not fibronectin
%A Manja Boehm
%A Benjamin Hoy
%A Manfred Rohde
%A Nicole Tegtmeyer
%A Kristoffer T B£¿k
%A Omar A Oyarzabal
%A Lone Br£¿ndsted
%A Silja Wessler
%A Steffen Backert
%J Gut Pathogens
%D 2012
%I BioMed Central
%R 10.1186/1757-4749-4-3
%X In the present study, we characterized the serine protease HtrA (high-temperature requirement A) of C. jejuni as a secreted virulence factor with important proteolytic functions. Infection studies and in vitro cleavage assays showed that C. jejuni¡¯s HtrA triggers shedding of the extracellular E-cadherin NTF domain (90 kDa) of non-polarised INT-407 and polarized MKN-28 epithelial cells, but fibronectin was not cleaved as seen for H. pylori¡¯s HtrA. Deletion of the htrA gene in C. jejuni or expression of a protease-deficient S197A point mutant did not lead to loss of flagella or reduced bacterial motility, but led to severe defects in E-cadherin cleavage and transmigration of the bacteria across polarized MKN-28 cell layers. Unlike other highly invasive pathogens, transmigration across polarized cells by wild-type C. jejuni is highly efficient and is achieved within a few minutes of infection. Interestingly, E-cadherin cleavage by C. jejuni occurs in a limited fashion and transmigration required the intact flagella as well as HtrA protease activity, but does not reduce transepithelial electrical resistance (TER) as seen with Salmonella, Shigella, Listeria or Neisseria.These results suggest that HtrA-mediated E-cadherin cleavage is involved in rapid crossing of the epithelial barrier by C. jejuni via a very specific mechanism using the paracellular route to reach basolateral surfaces, but does not cleave the fibronectin receptor which is necessary for cell entry.Infections with pathogenic food-borne bacteria constitute one of the leading causes of morbidity and mortality in humans. The World Health Organization (WHO) suggests that the human population worldwide suffers from about 4.5 billion incidences of gastroenteritis annually, causing approximately 1.8 million deaths [1]. Various Campylobacter species have been identified as the leading enteric bacterial infection worldwide [2,3]. Campylobacter jejuni is considered as a classical zoonotic pathogen, as it is found in
%K HtrA
%K E-cadherin
%K Fibronectin
%K MKN-28
%K Molecular pathogenesis
%K Cellular invasion
%K Signaling
%K TER
%K Virulence
%U http://www.gutpathogens.com/content/4/1/3