%0 Journal Article
%T Use of REP- and ERIC-PCR to reveal genetic heterogeneity of Vibrio cholerae from edible ice in Jakarta, Indonesia
%A Diana E Waturangi
%A Ignasius Joanito
%A Yogiara Yogi
%A Sabu Thomas
%J Gut Pathogens
%D 2012
%I BioMed Central
%R 10.1186/1757-4749-4-2
%X Seventy-five isolates of V. cholerae were recovered from ice samples collected from different locations of Jakarta. Specifically, 19 of them were identified as O1 serotype, 16 were Ogawa, 3 isolates were Inaba and the remaining isolates were non-O1. The fingerprinting profiles of V.cholerae isolated from ice samples were very diverse.This result showed that the ERIC sequence is more informative and discriminative than REP sequence for analysis of V. cholerae diversity.Cholera is characterized by severe watery diarrhea caused by toxigenic Vibrio cholerae, which colonizes the small intestine and produces an enterotoxin, the cholera toxin (CT).V. cholerae is classified on the basis of somatic antigens (O) into serovars or serogroups, and there are at least 200 known serogroup. Two serogroups, O1 and O139, have been associated with epidemic disease [1]. Serogroup O1 thought to include all the strains responsible for epidemic and endemic cholera; it has two major serotypes, Ogawa and Inaba. Each of those serotypes can be further divided into two biotypes, classical and El Tor, based on biochemical properties and susceptibility to bacteriophages. Serogroup O139 appears to be a hybrid of O1 strains and non-O1 strains. However, this organism does not produce O1 LPS and lacks at least some of the genetic material necessary for production of O1 antigen [2].In Indonesia, a total of 17 episodes of epidemic diarrheal disease were investigated from 1993 to 1999 and were found to be caused by V. cholerae O1 [3]. According to WHO report [4], there was a sharp increase in the number of cholera cases. A total of 131943 cases, including 2272 deaths, reported from 52 countries. Overall, this represents a 30% increase compared with the number of cases reported in 2004.In Indonesia edible are often used in street food and are consumed almost every day. Although it is so commonly consumed, it may not be prepared properly. We suspect that it may be a major important concern and we conducte
%K Vibrio cholerae
%K ERIC-PCR
%K REP-PCR
%K Edible ice
%U http://www.gutpathogens.com/content/4/1/2