%0 Journal Article
%T An in vitro study of osteoblast vitality influenced by the vitamins C and E
%A Kent Urban
%A Hans J H£¿hling
%A Beate L¨¹ttenberg
%A Thomas Szuwart
%A Ulrich Plate
%A Biomineralisation Research Unit
%J Head & Face Medicine
%D 2012
%I BioMed Central
%R 10.1186/1746-160x-8-25
%X In the present study we evaluate the effect of ascorbic acid (vitamin C) and ¦Á-tocopherol (vitamin E) on the proliferation and differentiation of primary bovine osteoblasts in vitro. Starting from standard growth medium we minimized the foetal calf serum to reduce their stimulatory effect on proliferation.An improved growth and an increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin was observed while increasing the ascorbic acid concentration up to 200 ¦Ìg/ml. Furthermore the effects of ¦Á-tocopherol on cell growth and cell differentiation were examined, whereby neither improved growth nor increased synthesis of the extracellular matrix proteins collagen type I, osteonectin and osteocalcin were detected.Further investigations are necessary to target at better supportive effect of vitamins on bone regeneration, and healing.Diseases like osteoporosis will become more and more a major public health threat in the near future, e.g. in the demographic aging trend. The prevention and an effective treatment against osteoporosis and other bone-associated diseases is therefore one of the aims in the field of medical research. Another challenge is the in vitro formation of bone in the field of tissue engineering.Proliferation and differentiation of osteoblasts enable the production of extracellular matrix (ECM) and is therefore the initial step to generate calcified tissue, especially bone. During the stages of differentiation, several proteins are synthesized by the osteoblasts, like collagen I, the main component of the ECM, and non-collagenous proteins like alkaline phosphatase, osteonectin and later in the differentiation progress osteocalcin. Up to now the scientific world focuses on the elucidation of the metabolic pathways during biomineralization to get an idea of how to promote the process of mineralization in vivo and in vitro. This could in the end lead to new applications in the coating of implants and prostheses or t
%K Bone cells
%K Osteoblast
%K Ascorbic acid
%K Tocopherol
%K Bone regeneration
%U http://www.head-face-med.com/content/8/1/25