%0 Journal Article
%T LIQUID CHROMATOGRAPHIC METHOD FOR THE SIMULTANEOUS QUANTITATIVE DETERMINATION OF CANDESARTAN CILEXETIL AND HYDROCHLORTHIAZIDE IN PHARMACEUTICAL DOSAGE FORMS
%A M. Mathrusri Annapurna
%A A. Narendra
%A K. Ravi Kumar
%J Journal of Drug Delivery and Therapeutics
%D 2012
%I Society of Pharmaceutical Technocrats
%X A simple and sensitive RP-HPLC method was developed and validated for the determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical dosage forms. The separation of components was achieved on a SHIMADZU Hypersil ODS-C18 column (250 ¡Á 4.6 mm, 5 ¦Ìm) with UV detection at 270 nm. Isocratic elution with a mobile phase consisting of 10 mM (pH 3.37) Tetra butyl ammonium hydrogen sulphate: methanol (15:85, V/V), at a flow rate 1.0 mL min-1was employed. Linearity was observed in the concentration range 0.625-62.5 ¦Ìg/mL for Hydrochlorthiazide and 0.8-80 ¦Ìg/mL for Candesartan cilexetil respectively. The linear regression equation was found to be Y=64002X-1412.6 for Hydrochlorthiazide and Y=24649X-6701.8 for Candesartan cilexetil respectively with correlation coefficients greater than 0.999. The LOD was found to be 0.1385 and 0.1892 ¦Ìg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively where as the LOQ was found to be 0.4394 and 0.6187¦Ìg/mL for Hydrochlorthiazide and Candesartan cilexetil respectively. The mean analytical recovery in determination of Candesartan cilexetil and Hydrochlorthiazide tablets was 99.31-100.08% Hydrochlorthiazide and 99.58-100.39% for Candesartan cilexetil respectively. Thus, the proposed method is applicable for routine determination of Candesartan cilexetil and Hydrochlorthiazide in pharmaceutical formulations.
%K Candesartan cilexetil
%K Hydrochlorthiazide
%K Liquid Chromatography
%K LOD
%K LOQ
%K Tablets
%U http://jddtonline.info/index.php/jddt/article/view/116/63