%0 Journal Article
%T Metallomic study of mercury in fish from Amazon region - Brazil
%A Moraes P. M.
%A Santos F. A.
%A Padilha C. F.
%A Castro G. R.
%J E3S Web of Conferences
%D 2013
%I 
%R 10.1051/e3sconf/20130126007
%X This paper presents preliminary findings for a metallomics study of mercury in the muscle of the fish from Amazonas region ¨C Brazil, after protein separation by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and subsequent evaluation of mercury by Thermal Decomposition and Amalgamation Coupled with Atomic Absorption (TTA-CAAS). It was found that mercury is present in 18 protein spots of ¡°dourada¡± muscle (Brachyplatystoma rousseauxii) and 9 protein spots of ¡°pacu¡± muscle (Myleussp.). The protein spots in which they were determined the presence of mercury present molecular weight of 13.5 and 21.4 kDa and isoelectric point of 3.8 and 9.2, respectively.
%K Mercury in fish
%K Metallomic study
%K 2D-PAGE
%K TTA-CAAS
%U http://dx.doi.org/10.1051/e3sconf/20130126007