%0 Journal Article
%T Optimization of extracellular mannanase production from Penicillium oxalicum KUB-SN2-1 and application for hydrolysis property
%A Sudathip Titapoka Chantorn
%A Katesarin Buengsrisawat
%A Apinya Pokaseam
%A Tasanee Sombat
%J Songklanakarin Journal of Science and Technology
%D 2013
%I Songkla University
%X Effects of media composition, and physical properties on the production of crude mannanase by Penicillium oxalicumKUB-SN2-1 were investigated. P.oxalicum KUB-SN2-1 was propagated in a shaking incubator at 30ˇăC with rotation speed of200 rpm of 7 days. The specific activity obtained during growth on robusta coffee residues (RCR) of 16.21 U/mg protein wasmuch higher than other carbon sources tested. For nitrogen sources, yeast extract (0.11 U/mg protein) and ammonium nitrate(0.09 U/mg protein) showed maximum specific activity. Hence, guar gum was the best inducer for producing mannanase (14U/mg protein). For evaluating the optimal concentration, the result showed that 1% guar gum, 0.5% yeast extract, 0.25%ammonium nitrate, and 0.25% RCR were the suitable sources of inducer, organic nitrogen, inorganic nitrogen, and carbon,respectively. Modified medium with initial culture pH of 5.0 at 30ˇăC was optimum for mannanase production (53.77 U/ml for3 day). Reducing sugars were analyzed by dinitrosalicylic acid methods. The highest reducing sugar of 7517.82  g/mlwas obtained from copra meal hydrolysate after 30 h.
%K Penicillium oxalicum
%K mannanase
%K copra meal
%K oligosaccharide production
%U http://rdo.psu.ac.th/sjstweb/journal/35-1/35-1-17-22.pdf