%0 Journal Article
%T Effect of increased copper ion content in the medium on the regeneration of androgenetic embryos of carrot (Daucus carota L.)
%A Urszula Kowalska
%A Katarzyna Szafra¨˝ska
%A Dorota KrzyŁżanowska
%A Waldemar Kiszczak
%J Acta Agrobotanica
%D 2012
%I Polish Botanical Society
%R 10.5586/aa.2012.060
%X The study was conducted to determine the effect of elevated concentrations of copper in the medium on the regeneration of androgenetic embryos of the carrot cultivar ˇ®Kazan F1ˇŻ obtained in anther cultures and to determine the level of soluble phenols produced in the regenerates under copper stress. Green embryos were laid out on 4 regeneration media based on B5 medium (G a m b o r g et al. 1968) without hormones, containing 0.1 ¨C control, 1, 10, and 100 ¦ĚM CuSO4ˇÁ5H2O. The plant material was passaged 3 times, after 4, 9 and 15 weeks. During these passages the emerging structures were examined; they were classified in terms of growth and development in vitro, weighed and counted. The levels of soluble phenols in the freeze-dried regenerates were determined. The elevated concentrations of copper in the regeneration media affected positively the formation of complete plants (rooted rosettes) and secondary embryos during the first 4 weeks of culture. After a longer regeneration time (9, 15 weeks), the elevated concentrations of copper caused negative effects: deformation of rosettes. After 15 weeks, the number of rooted rosettes decreased. The 9-week culture subjected to copper stress brought about an increase in the amounts of soluble phenols. The highest values were recorded in the rosettes treated with 10 ¦ĚM CuSO4. Prolonged exposure to media containing elevated concentrations of CuSO4 caused a reduction in the accumulation of phenolic compounds in the rosettes.
%K CuSO4
%K deformation/deformities
%K phenols
%K in vitro
%K rosettes
%K stress
%U http://pbsociety.org.pl/journals/index.php/aa/article/view/1307