%0 Journal Article
%T Liquid Chromatographic Determination of Pioglitazone in Pharmaceuticals, Serum and Urine Samples
%A Kamran Abro
%A Najma Memon
%A M.I. Bhanger
%A S.A. Mahesar
%J Pakistan Journal of Analytical and Environmental Chemistry
%D 2011
%I University of Sindh
%X A rapid and reliable analytical method based on high-performance liquid chromatography (HPLC) with UV detection (221nm) has been developed for the determination of the anti-hyperglycemic agent Pioglitazone in pharmaceutical formulations and biological fluids (serum and urine) after clean-up with solid-phase extraction. Chromatographic separation was achieved with a Chromolith  Performance RP-18e (100¡Á4.6mm) column using mobile phase composition of acetonitrile: mixed phosphate buffer (pH 2.5; 10mM) (30:70, v/v) with a flow rate of 2.0mL/min. The total run time was 2 min. under optimized conditions. The calibration curve was found to be linear in the range of 1-10 ¦Ìg mL-1 with regression coefficient of 0.9996, and the lower limit of detection 72 ng/20¦ÌL injection. The method has been validated for the system suitability, linearity, precision and accuracy, limits of detection, specificity, stability and robustness. The %recovery of Pioglitazone in pharmaceutical formulations was found to be 104.7%. The assay has been applied successfully to the pharmaceutical Tablet samples and biological fluids (serum and urine) of healthy volunteers.
%K Pioglitazone
%K Monolith column
%K Pharmaceuticals
%K Biological fluids.
%U http://ceacsu.edu.pk/PDF%20file/Volume%2012%20No%201%20and%202/49-54.pdf