%0 Journal Article
%T The Effect of Non-Coherent Impulse Radiation on Functional Status of Mononuclear Cells in Experiment
%A Е.V. Arkhipova
%A I.P. Ivanova
%J Sovremennye Tehnologii v Medicine
%D 2013
%I Nizhny Novgorod State Medical Academy of the Ministry of Health and Social Development
%X The aim of the investigation was to study the effect of non-coherent impulse radiation on functional status of mononuclear cells in experiment. Materials and Methods. In vivo experiments were carried out on white outbred male rats exposed to non-coherent impulse radiation with the following set-up parameters: impulse time ！ 10 μs, amperage ！ 1 kA, electrode voltage ！ 10 kV, pulse energy ！ 5 J, frequency ！ 1 Hz. We used two exposure modes on animals: three times within a minute, and three times within 2 min. We studied the state of oxygen-dependent neutrophil metabolism using spontaneous and induced NBT-test (NBT ！ nitro blue tetrazolium), assessed the activity of phagocytosis by latex particles phagocytosis, and determined spectrophotometrically the concentration of nucleic acids in lymphocytes of peripheral blood.Results. One-minute exposure caused no significant changes of functional status of mononuclear cells. Two-minute exposure resulted in NADPH-oxidase activation in neutrophil plasma membrane. Cell phagocytic rate was found to increase when the animals were exposed to non-coherent impulse radiation. Phagocytic index and phagocytic number increased of 21.84 and 45.28% respectively. There was revealed the increase of DNA concentration in lymphocytes of peripheral blood in rats.
%K Mononuclear cells
%K Phagocytosis
%K Neutrophil NADPH-oxidase
%K DNA
%K RNA
%K Non-coherent plasma radiation
%U http://www.stm-journal.ru/_resources/item/906/fields/File/stm_1_2013_s4_p27_31_eng.pdf