%0 Journal Article
%T Gentamicin supplemented polyvinylidenfluoride mesh materials enhance tissue integration due to a transcriptionally reduced MMP-2 protein expression
%A Marcel Binneb£¿sel
%A Klaus T von Trotha
%A Christina Ricken
%A Christian D Klink
%A Karsten Junge
%A Joachim Conze
%A Marc Jansen
%A Ulf P Neumann
%A Petra Lynen Jansen
%J BMC Surgery
%D 2012
%I BioMed Central
%R 10.1186/1471-2482-12-1
%X A PVDF mesh material was surface modified by plasma-induced graft polymerization of acrylic acid (PVDF+PAAc). Three different gentamicin concentrations were bound to the provided active sites of the grafted mesh surfaces (2, 5 and 8 ¦Ìg/mg). 75 male transgenic MMP-2/LacZ mice harbouring the LacZ reporter gene under control of MMP-2 regulatory sequence -1241/+423, excluding the RE-1 were randomized to five groups. Bilateral of the abdominal midline one of the five different meshes was implanted subcutaneously in each animal. MMP-2 gene transcription (anti-£¿-galactosidase staining) and MMP-2 protein expression (anti-MMP-2 staining) were analyzed semiquantitatively by immunohistochemistry 7, 21 and 90 days after mesh implantation. The collagen type I/III ratio was analyzed by cross polarization microscopy to determine the quality of mesh integration.The perifilamentary £¿-galactosidase expression as well as the collagen type I/III ratio increased up to the 90th day for all mesh modifications, whereas no significant changes could be observed for MMP-2 protein expression between days 21 and 90. Both the 5 and 8 ¦Ìg/mg gentamicin group showed significantly reduced levels of £¿-galactosidase expression and MMP-2 positive stained cells when compared to the PVDF group on day 7, 21 and 90 respectively (5 ¦Ìg/mg: p < 0.05 each; 8 ¦Ìg/mg: p < 0.05 each). Though the type I/III collagen ratio increased over time for all mesh modifications significant differences to the PVDF mesh were only detected for the 8 ¦Ìg/mg group at all 3 time points (p < 0.05 each).Our current data indicate that lack of RE-1 is correlated with increased mesh induced MMP-2-gene expression for coated as well as for non-coated mesh materials. Gentamicin coating reduced MMP-2 transcription and protein expression. For the 8 ¦Ìg/mg group this effect is associated with an increased type I/III collagen ratio. These findings suggest that gentamicin is beneficial for tissue integration after mesh implantation, which possib
%K mesh
%K gentamicin
%K PVDF
%K matrix metalloproteinase 2
%K wound healing
%U http://www.biomedcentral.com/1471-2482/12/1