%0 Journal Article
%T Promoters of Cancer Genes for Recombinant Protein Expression in Human Cancer Cell Lines
%A Mohammad Pourhassan-Moghaddam
%A Behrouz Farhadi
%A Kazem Nejati-Koshki
%A Tamouchin Moharrami
%J BioImpacts
%D 2012
%I Tabriz University of Medical Sciences
%X Introduction: Production of complex human recombinant proteins is an important issue in medical biotechnology. These proteins are mostly expressed in non-human mammalian host cells. This has some problems including non-human post-translational modifications, application of high-cost agents for inducing protein expression and low yields. Therefore, it is necessary to use new expression systems to overcome the indicated challenges. Methods: In this paper, we hypothesize the application of promoter regions of cancer genes, which have a high rate of transcription in human cancer cell lines, for designing new expression vectors. Results: After designing, these vectors could be applied to produce complex human recombinant proteins in the human cancer cell lines as production hosts. Conclusion: Application of these expression vectors for the production of recombinant human proteins in the human cancer cell lines have some advantages including authentic post-translational modifications, proper-cost of commercialization, and high yields.
%K Recombinant Protein Expression
%K Promoters
%K Cancer Genes
%K Human Cancer Cell Lines
%U http://dx.doi.org/10.5681/bi.2012.007