%0 Journal Article
%T Mayor actividad de rho kinasa en leucocitos circulantes se asocia a estr谷s oxidativo y rigidez arterial en hipertensos diab谷ticos Increased Rho-kinase activity in peripheral leukocytes is associated to oxidative stress and decreased compliance of the arterial wall in diabetic hypertensive patients
%A Luigi Gabrielli
%A Alejandro Berkovitz
%A Italo Mora
%A Ulises Novoa
%J Revista Chilena de Cardiolog赤a
%D 2011
%I Sociedad Chilena de Cardiolog赤a y Cirug赤a Cardiovascular
%X Introducci車n: La v赤a intracellular de RhoA/Rho kinasa es activada por agonistas de receptores acoplados a prote赤nas G peque as unidas a membrana. Su activaci車n est芍 relacionada al remodelado cardiovascular patol車gico. Previamente hemos observado aumento de actividad de Rho kinasa (ROCK) en pacientes con hipertensi車n arterial (HT) e hipertrofia ventricular izquierda como da o de 車rgano blanco. Pero su activaci車n en relaci車n a la diabetes no ha sido explorada en estos pacientes. Objetivo: Evaluar activaci車n de Rho kinasa y par芍metros de estr谷s oxidativo en pacientes hipertensos con diabetes tipo II (DMII). M谷todos: Estudio comparativo entre pacientes con HT sin tratamiento, HT con DMII y hemoglobina glicosi-lada Alc > 7,5% y un grupo control normotenso. Se realiz車 ecocardiograma de superficie. Se midi車 activaci車n de ROCK en leucocitos circulantes midiendo MYPT1 fosforilado/total (p/t) por Western blot y la velocidad de pulso carot赤deo-femoral (PWV) para estimar distensibilidad arterial. El stress oxidativo se estim車 midiendo ma-londialdeh赤do (MDA) y 8-isoprostano (8-ISO) en suero. Resultados: Se incluyeron 21 pacientes hipertensos con DMII, 38 pacientes hipertensos sin DMII y 34 controles normotensos. La edad promedio fue 51 ㊣ 0,9; 48 ㊣ 0,9 y 52 (p: NS) ㊣ 1,1 y el 47%, 50% y 52% (p: NS) eran mujeres respectivamente. Los pacientes HT con DMII presentaron MYPTl p/t (5,6 ㊣ 1,3; 3,6 ㊣ 0,4; 2,1 ㊣ 0,1 p< 0,01), MDA (1,8 ㊣ 0,4/Background: Rho/Rho-kinase intracellular pathway is activated by membrane bound small G-proteins. Activation of Rho/Rho-kinase pathway is related to pathologic cardiac remodeling. We have previously observed this activation (ROCK) in hypertensive patients with left ventricular hypertrophy. The influence of diabetes in this relationship has not been explored. Aim: to evaluate the activation of Rho-kinase and oxi-dative stress in hypertensive patients with type II diabetes (DMII). Methods: A comparative study between patients with untreated hypertension (HT), hypertensive patients with diabetes and hemoglobin A1c > 7.5% and normotensi-ve control subjects was performed. LVH was assessed by echocardiography. ROCK activity was measured in peripheral leukocytes by Western blot determination of phosphorilated / total MYPT1 ratio. Arterial compliance was determined by the relationship of carotid and femoral velocity signals (PWV) Oxidative stress was estimated by serum levels of malondialdehyde (MDA) and 8-isoprostane (8-ISO). Results: Hypertensives with DMII (n=21) had a mean age of 51 ㊣ 0.9 years, and 47% were females. Corresponding figures for
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