%0 Journal Article
%T Identification of immunogenic proteins and generation of antibodies against Salmonella Typhimurium using phage display
%A Torsten Meyer
%A Thomas Schirrmann
%A Andre Frenzel
%A Sebastian Miethe
%A Janin Stratmann-Selke
%A Gerald F Gerlach
%A Katrin Strutzberg-Minder
%A Stefan D¨ıbel
%A Michael Hust
%J BMC Biotechnology
%D 2012
%I BioMed Central
%R 10.1186/1472-6750-12-29
%X A phage display library of the entire Salmonella Typhimurium genome was constructed and 47 immunogenic oligopeptides were identified using a pool of convalescent sera from pigs infected with Salmonella Typhimurium. The corresponding complete genes of seven of the identified oligopeptids were cloned. Five of them were produced in E. coli. The immunogenic character of these antigens was validated with sera from pigs infeced with S. Tyhimurium and control sera from non-infected animals. Finally, human antibody fragments (scFv) against these five antigens were selected using antibody phage display and characterised.In this work, we identified novel immunogenic proteins of Salmonella Typhimurium and generated antibody fragments against these antigens completely based on phage display. Five immunogenic proteins were validated using a panel of positive and negative sera for prospective applications in diagnostics of Salmonela Typhimurium.
%U http://www.biomedcentral.com/1472-6750/12/29/abstract