%0 Journal Article
%T New cell line development for antibody-producing Chinese hamster ovary cells using split green fluorescent protein
%A Yeon-Gu Kim
%A Byoungwoo Park
%A Jung Oh Ahn
%A Joon-Ki Jung
%A Hong Weon Lee
%A Eun Gyo Lee
%J BMC Biotechnology
%D 2012
%I BioMed Central
%R 10.1186/1472-6750-12-24
%X An internal ribosome entry site (IRES) was introduced for using two green fluorescence protein (GFP) fragments as a reporter to both antibody chains, the heavy chain and the light chain. The cells co-transfected with two GFP fragments showed the emission of green fluorescence by the reconstitution of split GFP. The FACS-sorted pool with GFP expression had a higher specific antibody productivity (qAb) than that of the unsorted pool. The qAb was highly correlated with the fluorescence intensity with a high correlation coefficient, evidenced from the analysis of median GFP and qAb in individual selected clones.This study proved that the fragment complementation for split GFP could be an efficient indication for antibody production on the basis of high correlation of qAb with reconstitution of GFP. Taken together, we developed an efficient FACS-based screening method for high antibody-producing CHO cells with the benefits of the split GFP system.
%K Split GFP
%K FACS
%K Cell line development
%K Antibody production
%K CHO cells
%U http://www.biomedcentral.com/1472-6750/12/24/abstract