%0 Journal Article
%T Evaluation of test-kits for the detection of Escherichia coli O157 in raw meats and cattle faeces.
%A Hilda Nyati
%A Annet Heuvelink
%A Caroliene Van Heerwaarden
%A Ans Zwartkruis
%J International Journal of Food Studies
%D 2012
%I ISEKI_Food Association (IFA)
%X Escherichia coli O157 detection limits in artificially contaminated beef and cattle faeces samples were determined using Dynabeads anti E. coli O157 immunomagnetic beads, VIDAS-UP, VIDAS-ICE, and real-time PCR (GeneDisc and LightCycler) systems. Dynabeads anti-E. coli O157 immunomagnetic separation (IMS) and the GeneDisc cycler were the most sensitive methods, and could detect an initial 1 CFU in 25g beef samples after 6h of incubation in modified tryptone soya broth with novobiocin (mTSB+n) or buffered peptone water (BPW). The VIDAS-UP method could detect an initial 10 CFU, while VIDAS-ICE and the LightCycler methods could only detect an initial 100 CFU. Higher detection rates were achieved with 18 hour incubations, where an initial 1 CFU in a 25g sample could be detected with all five methods. For cattle faeces enrichments, Dynabeads anti-E. coli O157 IMS could detect an initial 1 CFU after a 6 h incubation in mTSB+n, while the VIDAS-UP and VIDAS-ICE methods could detect an initial 10 CFU and both PCR methods could only detect an initial 100 CFU. Detection rates were lower in BPW, compared to mTSB+n, with thresholds of 100 CFU for VIDAS-ICE, VIDAS-UP and GeneDisc methods, and >100 CFU for the LightCycler method.
%K E. coli O157
%K Dynabeads
%K VIDAS- ICE
%K VIDAS-UP
%K GeneDisc
%K LightCycler
%U http://www.iseki-food-ejournal.com/ojs/index.php/e-journal/article/view/49