%0 Journal Article
%T Laboratory capacity for diagnosis of foot-and-mouth disease in Eastern Africa: implications for the progressive control pathway
%A Alice Namatovu
%A Sabenzia Nabalayo Wekesa
%A Kirsten Tj£¿rneh£¿j
%A Moses Tefula Dhikusooka
%A Vincent B Muwanika
%A Hans Redlef Siegsmund
%A Chrisostom Ayebazibwe
%J BMC Veterinary Research
%D 2013
%I BioMed Central
%R 10.1186/1746-6148-9-19
%X The questionnaire response rate was 13/14 (93%). Twelve out of the 13 countries/regions had experienced at least one outbreak in the relevant five year period. Only two countries (Ethiopia and Kenya) had laboratories at biosecurity level 3 and only three (Ethiopia, Kenya and Sudan) had identified FMD virus serotypes for all reported outbreaks. Based on their own country/region assessment, 12/13 of these countries /regions were below stage 3 of the PCP-FMD. Quarantine (77%) and vaccination (54%) were the major FMD control strategies employed. The majority (12/13) of the NRLs used serological techniques to diagnose FMD, seven used antigen ELISA and three of these (25%) also used molecular techniques which were the tests most frequently requested from collaborating laboratories by the majority (69%) of the NRLs. Only 4/13 (31%) participated in proficiency testing for FMD. Four (31%) laboratories had no quality management systems (QMS) in place and where QMS existed it was still deficient, thus, none of the laboratories had achieved accreditation for FMD diagnosis.This study indicates that FMD diagnostic capacity in Eastern Africa is still inadequate and largely depends on antigen and antibody ELISAs techniques undertaken by the NRLs. Hence, for the region to progress on the PCP-FMD, there is need to: implement regional control measures, improve the serological diagnostic test performance and laboratory capacity of the NRLs (including training of personnel as well as upgrading of equipment and methods, especially strengthening the molecular diagnostic capacity), and to establish a regional reference laboratory to enforce QMS and characterization of FMD virus containing samples.Foot-and-mouth disease (FMD) is a highly contagious, acute, vesicular disease of cloven-hoofed domestic and wild animals [1]. The disease poses significant constraints through reduced productivity and limitation of international trade in live animals and their products [2,3]. The causal agent, foo
%U http://www.biomedcentral.com/1746-6148/9/19